Journal: PLoS ONE

Article Title: Copper and Zinc Interactions with Cellular Prion Proteins Change Solubility of Full-Length Glycosylated Isoforms and Induce the Occurrence of Heterogeneous Phenotypes

doi: 10.1371/journal.pone.0153931

Figure Lengend Snippet: Protein suspensions from brain homogenates derived from C57BL wild-type mice were reacted with increasing concentrations of ZnCl 2 , CuCl 2 and MgCl 2 as indicated. Proteins were centrifuged resulting in a separation of PrP C into a fraction of high solubility in the supernatant and a fraction of low solubility in the pellet. Following SDS-PAGE and immunoblotting, PrP C signals were visualized using mabs SAF34 (A) and SAF70 (B) followed by chemiluminescence substrate development. PrP C specific bands are indicated with d (diglycosylated), m (monoglycosylated), n (nonglycosylated) and C1 (truncated glycosylated C1 fragment). Neuron-specific enolase (NSE) was used as control (C).

Article Snippet: The brain marker neuron specific enolase (NSE) was used as a control protein detected by using a monoclonal mouse anti-NSE antibody, which was purchased as a purified IgG antibody (Dianova, Hamburg, Germany).

Techniques: Derivative Assay, Solubility, SDS Page, Western Blot

Journal: Fertility and sterility

Article Title: FACS and MACS sorting strategies to isolate and enrich human spermatogonial stem cells

doi: 10.1016/j.fertnstert.2014.04.036

Figure Lengend Snippet: Immunofluorescence co-staining for SALL4 and ZBTB16 (A–D), SALL4 and KIT (E–H), UTF1 and SALL4 (I–L), UTF1 and KIT (M–P), UTF1 and UCHL1 (Q–T), UCHL1 and KIT (U–X), UCHL1 and ENO2 (Y-BB) and SALL4 and ENO2 (CC–FF) in adult human testis. DAPI staining (blue) identifies all the nuclei. The bar graphs show quantification and relative proportion of each co-staining. The quantification is shown as the mean number of positive cells per cross-section of a seminiferous tubule. At least 100 seminiferous tubules were counted from 3 different organ donors. Bar graphs in D, H, L, P, T, X and BB indicate the mean percentage of marker positive cells. Error bars represent SEM. Scale bars = 100 μm.

Article Snippet: Subsequently, sections were stained for 90 minutes at room temperature with the following primary antibodies in antibody diluent: mouse anti-UTF1 (1:50, MAB4337, Millipore) goat anti-ZBTB16 (1:50; AF2944, R&D Systems), rabbit anti- KIT; goat anti-KIT (1:400; A4502, DakoCytomation; 1:50; AF332, R&D Systems), rabbit anti-SALL4 (1:500; ab29112, Abcam; 1:40; ab181087, Abcam), mouse anti-ENO2 (1:500, LS-B2890, LSBio), rabbit anti-UCHL1 (1:1000, 7863-0507, Biogenesis), rabbit anti-EPCAM (1:200; ab71919, Abcam), rabbit anti-ITGA6 (1:100; ab75737, Abcam).

Techniques: Immunofluorescence, Staining, Marker